Leica microsystems heidelberg gmbh lcs software functions user manual leica lcs english page 8 of 164 art. The leica sp8 5d is equipped with a series of five tuneable photoreceptors. With the filterfree spectral detection system of the leica tcs sp8, you can simultaneously record up to 5 different dye emissions coming from your specimen without any loss. Upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs. User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. For such experiments, live data mode is the tool of choice. Turn on the fluorescent lamp top left of the computer monitor. Together with an aobs acoustical optical beam splitter up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. The microscope is suitable for both live cell and fixed specimens on slides, and it will run alongside the old zeiss lms 510 metahead confocal.
You must have additional training to use the advanced features on this system. Free download books leica sp8 manual printable 2019 you know that reading leica sp8 manual printable 2019 is beneficial, because we are able to get enough detailed information online through the resources. This user manual specifies names of products or services that. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. If you do not require high speed scanning leave resonant set to.
This user manual provides you with important information for using the system, the necessary ambient conditions and the usable lasers. Leica m9 m9p camera user manual, instruction manual, user guide pdf free download leica m9 m9p pdf user manual, user guide, instructions, leica m9 m9p owners manual. Technology has developed, and reading leica sp8 manual printable 2019 books can be more convenient and much easier. User manuals, guides and specifications for your leica dmi6000b microscope. Contents startup preparing for imaging part 1 on the scope. Quick operation manual for leica sp8 confocalmultiphoton microscope.
Continuously tunable excitation and detection ensure best image quality and sample protection. Solid state lasers and the el6000 widefield light source require only minimal warm up, but adhere to 15min15min rule. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multi photon confocal microscopy. Tcs sp8 mp with dmi6000 and ir laser without dispersion compensation. Contact us for any other documents regarding this product. This screen highlights the aperture opening and the intensity of the transmitted light. The sp8 lightning allows you to perform proper sampling over a specimen area at least six times larger than with any other confocal system.
No 159330033 vers 01082001 click on an item means to move the pointer onto the specified item and press and release the mouse button once. View and download leica tcs sp8 smd user manual online. Leica sp8 tcs users manual v2 university of oklahoma. Leica sp8 the leica microsystems sp8 confocal with digital light sheet has the following features and capabilities. Double click on the las x software found on the desktop. Then turn on the power supply for the scanner 1 and the lasers 2 by. Check to make sure antivibration table is on power switch in the back of unit 3. Oct 21, 2017 leica sp8 pointscanning confocal system, on a leica dmi6000b inverted microscope, confocal laser scanning microscope leica tcs sp8 en, pdf, 535. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. Technology has developed, and reading leica sp8 manual printable 2019 books might be more convenient and simpler. Leica sp8 dive multiphoton microscope manualquick guide. Usermanual for leica sp8 confocal contact information. Turn on the ctr control box, fluorescent light for the microscope stand.
The leica tcs sp8 is designed to meet all of your future requirements with effectiveness and efficiency. Leica sp8 manual printable 2019 read online at permacultureph. Database contains 3 leica dmi6000b manuals available for free online viewing or downloading in pdf. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser.
Leica microsystems cms gmbh am friedensplatz 3 d68165 mannheim germany. Leica sp8 manual printable 2019 books might be more convenient and simpler. Leica tcs sp8 x the only freely tunable confocal system the white light laser source of the leica tcs sp8 x perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Instructions for using leica dcm3d confocal microscope. The leica sp8x smd confocal microscope is located in room 00075, lab 1. The microscope is suitable for both live cell and fixed specimens on slides, and it will. Leica sp8 resonant confocal nrimcdb microscopy facility. User manual leica tcs sp8 smd for fcs, flim and flcs.
Listed below are some websites for downloading free pdf books where you can acquire the maximum amount of. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018. The aobs, a uniquely flexible beam splitter, makes it possible to acquire spectrally close fluorophores like. In addition, this operating manual provides a rough overview of the. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and. Adjust the laser intensity of the appropriate laser. Confocal imaging using a super continuum, white light wll pulsed laser source that allows users to specify any excitation wavelength between 470nm and 670nm. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. Hence, there are several books entering pdf format. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell imaging mode and light sheet. The leica microsystems sp8 confocal with digital light sheet has the following features and capabilities. Enable the lasers by turning the key from the off position.
It achieves this level of performance using highspeed resonant scanning with imaging formats of up to 2496 x 2496 pixels. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. If you do not require high speed scanning leave resonant set to off. Live cell imaging or light sheet mode that exceeds a 4hour duration must be conducted at. Leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options specifications antivibration table yes activepassive microscope stage stage options specifications autofocus optional reflectionbased adaptive focus control afc for leica dmi8 cs with. User guide to the ibif leica tcs sp8 mp confocal microscope. Turn on power to microscope power unit is located at your feet, next to the computer. Sp8 lightning confocal microscope products leica microsystems. Turn on the ctr control box, el6000fluorescent light source for the. May 18, 2016 leica m9 m9p camera user manual, instruction manual, user guide pdf free download leica m9 m9p pdf user manual, user guide, instructions, leica m9 m9p owners manual. Sp8 lightning confocal microscope leica microsystems.
A guide through the live data mode live data mode with leica tcs sp8 5 3 definition of an experiment some experiments can be more complex and require differ ent hardware settings at a certain time within the course of the experiment. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy. Microscopes microscope stand options upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options speci. This user manual specifies names of products or services that are trademarks or. View and download leica tcs sp8 mp user manual online. Never again compromise on speed, resolution and sensitivity. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk.
Las x live data mode recording of manual and automated workflows, trigger functions, complex timelapse series. Triggering with tcssp8 and dm6000 cfs en, pdf, 666 kb. Define upper and lower emission band thresholds based on the spectrum for your fluorophore. Sp8 lightning is a modular confocal microscope designed for optimal. Leica m9 m9p is the mmount fullformat digital rangefinder camera equipped with a 18 mp ccd sensor developed specifically for this camera, enables the capture of. In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell. View and download leica sp8 training manual online. View and download leica tcs sp8 quick start manual online. Tcs sp8 smd laboratory equipment pdf manual download.
Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. The gain setting as discussed elsewhere in this manual controls the sensitivity. Adjust the laser intensity of the appropriate laser lines by moving. Turning on the system 1turn on the fluorescent light source number 1 2 turn on the ndd power supply. User guide created by daniel desantis and linda mayernik, october, 2015. Operational training manual sp8 quick start instructions. They prefer to invest their idle time to talk or hang out. The leica sp8 x smd confocal microscope is located in room 00075, lab 1. It is built around the leica prismbased spectral detector system on a motorized inverted microscope stand. Quick operation manual for leica sp8 confocalmultiphoton. Leica sp8 pointscanning confocal system, on a leica dmi6000b inverted microscope, confocal laser scanning microscope leica tcs sp8 en, pdf, 535.
Adjust the laser intensity of the appropriate laser lines by moving the slider up or by directly. We can read books on the mobile, tablets and kindle, etc. Continuously tunable excitation and detection ensure best. This user manual specifies names of products or services that are trademarks or registered. School of life sciences these are procedures provided by leica microsystems that describe how to operate the instrument in its most simplest format. Training guide for leica sp8 confocalmultiphoton microscope. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained. Usermanual for leica sp8 confocal universitetet i bergen. Leica tcs sp8 technical documentation life science research division. Leica sp8 manual printable 2019great ebook you must read is leica sp8 manual printable 2019. Free download books leica sp8 manual printable 2019 everyone knows that reading leica sp8 manual printable 2019 is beneficial, because we could get a lot of information in the resources.
999 1381 443 1513 1239 102 1503 138 946 732 775 1396 1351 102 1330 670 978 1578 1325 1134 1241 1456 820 944 751 167 1053 298 1551 1287 924 1252 680 177 1270 881 353 329 1187 29 155